Characterization of SlFSR Gene for Increasing the Shelf Life of Tomato by Some Bioinformatics Tools

Abstract

Tomatoes are known as Solanum lycopersicum in scientific language around the world. These are predominantly consumed in the cooking of food and salads. It enhances the food's taste. Due to being temperature sensitive, it has a short life span on the kitchen shelf. To control the temperature sensitivity of tomatoes, first understand the mechanism of the genes that are involved in it. This study is mainly related to the SlFSR gene. It is involved in the ripening mechanism that’s why it has a great impact in controlling the shelf life of tomatoes. The GRAS transcriptional factor controls many gene’s process, it is also responsible for the proper functioning of SlFSR gene. The genes of the GRAS (TF) family contribute its role in the ripening procedure of fruits. To increase the shelf life of fruits, these genes will be helpful. According to this study, If SlFSR gene mute its function, or slow down the process of ripening it may enhance the shelf life of the fruit. This Insilico-based research, made the understanding of different computational analysis. It provides the deep information about the target gene, e.g., structure, function, and atomic composition of the gene. The Expasy translate tool is used to convert nucleotide sequence into protein sequence, Expasy Portparam identifies the domain and function of the gene, InterProScan tells about the protein family, Swiss model is used to generate the 3-D modeling structure, and the phylogeny tree is generated by ClustalW tool to understand the evolutionary history of the desired gene. The insilico tools and softwares predict that the SlFSR gene product has instability index of 48.52, 89.77 aliphatic indexes, and a -0.245 Grand average of hydropathicity. These values strongly predict that the SlFSR is unstable and belongs to the transcription GRAS family that may alter the expression of the genes for the desired product by using DNA binding protein.